(eng): Many diagnostic methods using scintigraphic image sequence require decomposition of the sequence into tissue images and their time-activity curves. Standard procedure for this task is still manual selection of regions of interest (ROIs) which can be highly subjective due to their overlaps and poor signal-to-noise ratio. This can be overcome by automatic decomposition, however, the results may not have good physiological meaning. In this contribution, we aim to combine these approaches in semi-supervised procedure which is based on Bayesian blind source separation with the possibility of manual interaction after each run until an acceptable solution is obtained. The manual interaction is based on manual ROI placement and using its position to modify the corresponding prior parameters of the model. Performance of the proposed method is studied on real scintigraphic image sequence as well as on estimation of the specific diagnostic parameter on representative dataset of 10 scintigraphic sequences.